William A. Beard Laboratory of Structural Biology NIEHS, National Institutes of Health Biography Publications Institution JoVE Articles William A. Beard has not added a biography. If you are William A. Beard and would like to personalize this page please email our Author Liaison for assistance. Publications Understanding the Loss-of-function in a Triple Missense Mutant of DNA Polymerase β Found in Prostate Cancer International Journal of Oncology. Oct, 2013 | Pubmed ID: 23877444 Observing a DNA Polymerase Choose Right from Wrong Cell. Jul, 2013 | Pubmed ID: 23827680 Insights into the Conformation of Aminofluorene-deoxyguanine Adduct in a DNA Polymerase Active Site The Journal of Biological Chemistry. Aug, 2013 | Pubmed ID: 23798703 Amino Acid Substitution in the Active Site of DNA Polymerase β Explains the Energy Barrier of the Nucleotidyl Transfer Reaction Journal of the American Chemical Society. May, 2013 | Pubmed ID: 23647366 DNA Polymerase Minor Groove Interactions Modulate Mutagenic Bypass of a Templating 8-oxoguanine Lesion Nucleic Acids Research. Feb, 2013 | Pubmed ID: 23267011 Perspective: Pre-chemistry Conformational Changes in DNA Polymerase Mechanisms Theoretical Chemistry Accounts. Nov, 2012 | Pubmed ID: 23459563 DNA Polymerase β Gap-filling Translesion DNA Synthesis Chemical Research in Toxicology. Dec, 2012 | Pubmed ID: 23121263 Effect of β,γ-CHF- and β,γ-CHCl-dGTP Halogen Atom Stereochemistry on the Transition State of DNA Polymerase β Biochemistry. Oct, 2012 | Pubmed ID: 23043620 DNA Sequence Context Effects on the Glycosylase Activity of Human 8-oxoguanine DNA Glycosylase The Journal of Biological Chemistry. Oct, 2012 | Pubmed ID: 22989888 Structures of DNTP Intermediate States During DNA Polymerase Active Site Assembly Structure (London, England : 1993). Nov, 2012 | Pubmed ID: 22959623 Stereospecific Formation of a Ternary Complex of (S)-α,β-fluoromethylene-dATP with DNA Pol β Chembiochem : a European Journal of Chemical Biology. Mar, 2012 | Pubmed ID: 22315190 Binary Complex Crystal Structure of DNA Polymerase β Reveals Multiple Conformations of the Templating 8-oxoguanine Lesion Proceedings of the National Academy of Sciences of the United States of America. Jan, 2012 | Pubmed ID: 22178760 Metal-induced DNA Translocation Leads to DNA Polymerase Conformational Activation Nucleic Acids Research. Apr, 2012 | Pubmed ID: 22169953 Molecular Insights into DNA Polymerase Deterrents for Ribonucleotide Insertion The Journal of Biological Chemistry. Sep, 2011 | Pubmed ID: 21733843 Substrate Channeling in Mammalian Base Excision Repair Pathways: Passing the Baton The Journal of Biological Chemistry. Dec, 2010 | Pubmed ID: 20952393 Base Excision Repair and Design of Small Molecule Inhibitors of Human DNA Polymerase β Cellular and Molecular Life Sciences : CMLS. Nov, 2010 | Pubmed ID: 20844920 Synthesis and Biological Evaluation of Fluorinated Deoxynucleotide Analogs Based on Bis-(difluoromethylene)triphosphoric Acid Proceedings of the National Academy of Sciences of the United States of America. Sep, 2010 | Pubmed ID: 20724659 DNA Polymerase Structure-based Insight on the Mutagenic Properties of 8-oxoguanine Mutation Research. Nov, 2010 | Pubmed ID: 20696268 Mutagenic Conformation of 8-oxo-7,8-dihydro-2'-dGTP in the Confines of a DNA Polymerase Active Site Nature Structural & Molecular Biology. Jul, 2010 | Pubmed ID: 20526335 DNA Polymerase Beta Ribonucleotide Discrimination: Insertion, Misinsertion, Extension, and Coding The Journal of Biological Chemistry. Aug, 2010 | Pubmed ID: 20519499 Halogenated Beta,gamma-methylene- and Ethylidene-dGTP-DNA Ternary Complexes with DNA Polymerase Beta: Structural Evidence for Stereospecific Binding of the Fluoromethylene Analogues Journal of the American Chemical Society. Jun, 2010 | Pubmed ID: 20465217 DNA Polymerase Beta Substrate Specificity: Side Chain Modulation of the "A-rule" The Journal of Biological Chemistry. Nov, 2009 | Pubmed ID: 19759017 DNA Polymerase Beta and PARP Activities in Base Excision Repair in Living Cells DNA Repair. Nov, 2009 | Pubmed ID: 19748837 A Real-time Fluorescence Method for Enzymatic Characterization of Specialized Human DNA Polymerases Nucleic Acids Research. Oct, 2009 | Pubmed ID: 19684079 Coordination Between Polymerase Beta and FEN1 Can Modulate CAG Repeat Expansion The Journal of Biological Chemistry. Oct, 2009 | Pubmed ID: 19674974 Alpha,beta-difluoromethylene Deoxynucleoside 5'-triphosphates: a Convenient Synthesis of Useful Probes for DNA Polymerase Beta Structure and Function Organic Letters. May, 2009 | Pubmed ID: 19351147 Alpha,beta-methylene-2'-deoxynucleoside 5'-triphosphates As Noncleavable Substrates for DNA Polymerases: Isolation, Characterization, and Stability Studies of Novel 2'-deoxycyclonucleosides, 3,5'-cyclo-dG, and 2,5'-cyclo-dT Journal of Medicinal Chemistry. Oct, 2008 | Pubmed ID: 18811136 Structures of DNA Polymerase Beta with Active-site Mismatches Suggest a Transient Abasic Site Intermediate During Misincorporation Molecular Cell. May, 2008 | Pubmed ID: 18471977 Negligible Impact of Pol Iota Expression on the Alkylation Sensitivity of Pol Beta-deficient Mouse Fibroblast Cells DNA Repair. Jun, 2008 | Pubmed ID: 18434259 Incorrect Nucleotide Insertion at the Active Site of a G:A Mismatch Catalyzed by DNA Polymerase Beta Proceedings of the National Academy of Sciences of the United States of America. Apr, 2008 | Pubmed ID: 18391201 DNA Polymerase Beta Fidelity: Halomethylene-modified Leaving Groups in Pre-steady-state Kinetic Analysis Reveal Differences at the Chemical Transition State Biochemistry. Jan, 2008 | Pubmed ID: 18161950 Exploring the Role of Large Conformational Changes in the Fidelity of DNA Polymerase Beta Proteins. Jan, 2008 | Pubmed ID: 17671961 (R)-beta,gamma-fluoromethylene-dGTP-DNA Ternary Complex with DNA Polymerase Beta Journal of the American Chemical Society. Dec, 2007 | Pubmed ID: 18031037 The X Family Portrait: Structural Insights into Biological Functions of X Family Polymerases DNA Repair. Dec, 2007 | Pubmed ID: 17631059 Coordination of Steps in Single-nucleotide Base Excision Repair Mediated by Apurinic/apyrimidinic Endonuclease 1 and DNA Polymerase Beta The Journal of Biological Chemistry. May, 2007 | Pubmed ID: 17355977 Differing Conformational Pathways Before and After Chemistry for Insertion of DATP Versus DCTP Opposite 8-oxoG in DNA Polymerase Beta Biophysical Journal. May, 2007 | Pubmed ID: 17293403 Modifying the Beta,gamma Leaving-group Bridging Oxygen Alters Nucleotide Incorporation Efficiency, Fidelity, and the Catalytic Mechanism of DNA Polymerase Beta Biochemistry. Jan, 2007 | Pubmed ID: 17209556 Magnesium-cationic Dummy Atom Molecules Enhance Representation of DNA Polymerase Beta in Molecular Dynamics Simulations: Improved Accuracy in Studies of Structural Features and Mutational Effects Journal of Molecular Biology. Feb, 2007 | Pubmed ID: 17174326 Regulation of DNA Repair Fidelity by Molecular Checkpoints: "gates" in DNA Polymerase Beta's Substrate Selection Biochemistry. Dec, 2006 | Pubmed ID: 17176036 Structure of DNA Polymerase Beta with a Benzo[c]phenanthrene Diol Epoxide-adducted Template Exhibits Mutagenic Features Proceedings of the National Academy of Sciences of the United States of America. Nov, 2006 | Pubmed ID: 17079493 Energy Analysis of Chemistry for Correct Insertion by DNA Polymerase Beta Proceedings of the National Academy of Sciences of the United States of America. Sep, 2006 | Pubmed ID: 16938895 Activities and Mechanism of DNA Polymerase Beta Methods in Enzymology. 2006 | Pubmed ID: 16793365 Determination of Lysine PK Values Using [5-13C]lysine: Application to the Lyase Domain of DNA Pol Beta Journal of the American Chemical Society. Jun, 2006 | Pubmed ID: 16787052 Magnesium-induced Assembly of a Complete DNA Polymerase Catalytic Complex Structure (London, England : 1993). Apr, 2006 | Pubmed ID: 16615916 Structure and Mechanism of DNA Polymerase Beta Chemical Reviews. Feb, 2006 | Pubmed ID: 16464010 A Thymine Isostere in the Templating Position Disrupts Assembly of the Closed DNA Polymerase Beta Ternary Complex Biochemistry. Nov, 2005 | Pubmed ID: 16285726 Syn-full Behavior by T7 DNA Polymerase Structure (London, England : 1993). Nov, 2005 | Pubmed ID: 16271879 Mismatch-induced Conformational Distortions in Polymerase Beta Support an Induced-fit Mechanism for Fidelity Biochemistry. Oct, 2005 | Pubmed ID: 16201758 Structural Insight into the DNA Polymerase Beta Deoxyribose Phosphate Lyase Mechanism DNA Repair. Dec, 2005 | Pubmed ID: 16172026 Nucleotide-induced DNA Polymerase Active Site Motions Accommodating a Mutagenic DNA Intermediate Structure (London, England : 1993). Aug, 2005 | Pubmed ID: 16084394 DNA Polymerase Lambda Mediates a Back-up Base Excision Repair Activity in Extracts of Mouse Embryonic Fibroblasts The Journal of Biological Chemistry. May, 2005 | Pubmed ID: 15749700 DNA Polymerase Beta and Flap Endonuclease 1 Enzymatic Specificities Sustain DNA Synthesis for Long Patch Base Excision Repair The Journal of Biological Chemistry. Feb, 2005 | Pubmed ID: 15561706 Structural Insights into DNA Polymerase Beta Deterrents for Misincorporation Support an Induced-fit Mechanism for Fidelity Structure (London, England : 1993). Oct, 2004 | Pubmed ID: 15458631 Dynamic Characterization of a DNA Repair Enzyme: NMR Studies of [methyl-13C]methionine-labeled DNA Polymerase Beta Biochemistry. Jul, 2004 | Pubmed ID: 15248749 Critical Role of Magnesium Ions in DNA Polymerase Beta's Closing and Active Site Assembly Journal of the American Chemical Society. Jul, 2004 | Pubmed ID: 15238001 Highly Organized but Pliant Active Site of DNA Polymerase Beta: Compensatory Mechanisms in Mutant Enzymes Revealed by Dynamics Simulations and Energy Analyses Biophysical Journal. Jun, 2004 | Pubmed ID: 15189842 Influence of DNA Structure on DNA Polymerase Beta Active Site Function: Extension of Mutagenic DNA Intermediates The Journal of Biological Chemistry. Jul, 2004 | Pubmed ID: 15145936 High-level Expression and Purification of Untagged and Histidine-tagged HIV-1 Reverse Transcriptase Protein Expression and Purification. Mar, 2004 | Pubmed ID: 14766302 Localization of the Deoxyribose Phosphate Lyase Active Site in Human DNA Polymerase Iota by Controlled Proteolysis The Journal of Biological Chemistry. Aug, 2003 | Pubmed ID: 12777390 Structural Insights into the Origins of DNA Polymerase Fidelity Structure (London, England : 1993). May, 2003 | Pubmed ID: 12737815 Structure of DNA Polymerase Beta with the Mutagenic DNA Lesion 8-oxodeoxyguanine Reveals Structural Insights into Its Coding Potential Structure (London, England : 1993). Jan, 2003 | Pubmed ID: 12517346 Rapid Segmental and Subdomain Motions of DNA Polymerase Beta The Journal of Biological Chemistry. Feb, 2003 | Pubmed ID: 12458221 Efficiency of Correct Nucleotide Insertion Governs DNA Polymerase Fidelity The Journal of Biological Chemistry. Dec, 2002 | Pubmed ID: 12370169 Polymerase Beta Simulations Suggest That Arg258 Rotation is a Slow Step Rather Than Large Subdomain Motions Per Se Journal of Molecular Biology. Apr, 2002 | Pubmed ID: 11955015 Loss of DNA Polymerase Beta Stacking Interactions with Templating Purines, but Not Pyrimidines, Alters Catalytic Efficiency and Fidelity The Journal of Biological Chemistry. Mar, 2002 | Pubmed ID: 11756435 Steady-state, Pre-steady-state, och Single-omsättningen Kinetic Mätning för DNA-glykosylas aktivitet Akira Sassa1, William A. Beard1, David D. Shock1, Samuel H. Wilson1 1Laboratory of Structural Biology, NIEHS, National Institutes of Health JoVE 50695 Chemistry
Steady-state, Pre-steady-state, och Single-omsättningen Kinetic Mätning för DNA-glykosylas aktivitet Akira Sassa1, William A. Beard1, David D. Shock1, Samuel H. Wilson1 1Laboratory of Structural Biology, NIEHS, National Institutes of Health JoVE 50695 Chemistry