Other Publications (1)
Articles by Yair Glick in JoVE
High-throughput Protein Expression Generator Using a Microfluidic Platform Yair Glick*1, Dorit Avrahami*1, Efrat Michaely1, Doron Gerber1 1The Mina & Everard Goodman Faculty of Life Sciences, The Nanotechnology Institute, Bar-Ilan University We present a microfluidic approach for the expression of protein arrays. The device consists of thousands of reaction chambers controlled by micro-mechanical valves. The microfluidic device is mated to a microarray-printed gene library. These genes are then transcribed and translated on-chip, resulting in a protein array ready for experimental use.
Other articles by Yair Glick on PubMed
Sperm Epidermal Growth Factor Receptor (EGFR) Mediates Î±7 Acetylcholine Receptor (AChR) Activation to Promote Fertilization The Journal of Biological Chemistry. Jun, 2012 | Pubmed ID: 22577141 To attain fertilization the spermatozoon binds to the egg zona pellucida (ZP) via sperm receptor(s) and undergoes an acrosome reaction (AR). Several sperm receptors have been described in the literature; however, the identity of this receptor is not yet certain. In this study, we suggest that the Î±7 nicotinic acetylcholine receptor (Î±7nAChR) might be a sperm receptor activated by ZP to induce epidermal growth factor receptor (EGFR)-mediated AR. We found that isolated ZP or Î±7 agonists induced the AR in sperm from WT but not Î±7-null spermatozoa, and the induced AR was inhibited by Î±7 or EGFR antagonists. Moreover, Î±7-null sperm showed very little binding to the egg, and microfluidic affinity in vitro assay clearly showed that Î±7nAChR, as well as EGFR, interacted with ZP3. Induction of EGFR activation and the AR by an Î±7 agonist was inhibited by a Src family kinase (SFK) inhibitor. In conclusion we suggest that activation of Î±7 by ZP leads to SFK-dependent EGFR activation, Ca(2+) influx, and the acrosome reaction.