In JoVE (3)
- Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization
- A Rat Model of Orthotopic Liver Transplantation Using a Novel Magnetic Anastomosis Technique for Suprahepatic Vena Cava Reconstruction
- A 3-dimensional (3D)-printed Template for High Throughput Zebrafish Embryo Arraying
Other Publications (1)
Articles by Yue Li in JoVE
Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization Christina N. Cheng1, Yue Li1, Amanda N. Marra1, Valerie Verdun1, Rebecca A. Wingert1 1Department of Biological Sciences, University of Notre Dame The zebrafish embryo is an excellent model for developmental biology research. During embryogenesis, zebrafish develop with a yolk mass, which presents three-dimensional challenges for sample observation and analysis. This protocol describes how to create two-dimensional flat mount preparations of whole mount in situ (WISH) stained zebrafish embryo specimens.
A Rat Model of Orthotopic Liver Transplantation Using a Novel Magnetic Anastomosis Technique for Suprahepatic Vena Cava Reconstruction Lifei Yang*1,2, Jianwen Lu*1,2,3, Yue Wang1,2,3, Mei Zhang1,2,3, Yuan Shi4, Shasha Wei1,2, Peng Liu1,2,3, Zheng Wu3, Yi Lv1,2,3, Rongqian Wu1,2 1 The reconstruction of the suprahepatic vena cava (SHVC) remains a difficult step in rat orthotopic liver transplantation. In this article, we show a step-by-step protocol for SHVC reconstruction in rats using a novel magnetic anastomosis technique.
A 3-dimensional (3D)-printed Template for High Throughput Zebrafish Embryo Arraying Tianyu Yu1,2, Yue Jiang1, Sijie Lin1 1College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, 2UNEP-Tongji Institute of Environment for Sustainable Development, Tongji University Here, we present a protocol to design and fabricate a zebrafish embryo arraying template, followed by a detailed procedure on the use of such template for high throughput zebrafish embryo arraying into a 96-well plate.
Other articles by Yue Li on PubMed
Rapid Diagnosis and Discrimination of Bacterial Meningitis in Children Using Gram Probe Real-Time Polymerase Chain Reaction Clinical Pediatrics. May, 2014 | Pubmed ID: 24790023 In this study, we developed a method of simultaneous detection and discrimination of bacteria in cerebrospinal fluid (CSF) with gram probe real-time polymerase chain reaction (PCR). Our results showed 25 clinical strains representing 13 gram-positive and 12 gram-negative bacterial species. They were identified correctly with the corresponding gram probe. The standard curve showed that the amplification efficiency of templates with different concentrations of bacteria was almost the same with a potential detection limit of 10 colony-forming units/mL. A total of 482 children who were clinically suspected of bacterial meningitis were included in this study. A total of 1.0 mL of CSF was collected from every child and was subjected to gram probe-based PCR (GP-PCR), CSF culture, and CSF routine analysis. The positive rate of the GP-PCR array was (32/482, 6.64%) significantly higher than that of CSF culture (23/482, 4.77%). GP-PCR was proved to be an excellent technique for rapid and accurate diagnosis and discrimination of bacterial meningitis, and hence its use as a diagnostic tool in future seems very promising.