Articles by Yves Molino in JoVE
Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport Yves Molino1, Françoise Jabès1, Emmanuelle Lacassagne2, Nicolas Gaudin2, Michel Khrestchatisky2 1VECT-HORUS SAS, 2Aix-Marseille Université, CNRS, NICN UMR 7259 The aim of the present study was to validate the reproducibility of an in vitro BBB model involving a rat syngeneic co-culture of endothelial cells and astrocytes. The endothelial cell monolayer presented high TEER and low LY permeability. Expression of specific TJ proteins, functional responses to inflammation and functionality of transporters and receptors were assessed.
Other articles by Yves Molino on PubMed
Spatial Confinement of Neurite Regrowth from Dorsal Root Ganglia Within Nonporous Microconduits Tissue Engineering. Apr, 2003 | Pubmed ID: 12740083 Tissue engineering is founded on the concept of controlling the behavior of individual cells to stimulate tissue formation. This control is achieved by mimicking signals that manage natural tissue development or repair. These interdependent signals include cytokine delivery, extracellular matrix interactions, and cell-cell communication. Here, we report on the effect of spatial guidance as a signal for nerve tissue regeneration, using a simple in vitro model. We observe the acceleration of neurite extension from rat dorsal root ganglia within micron-scale tubes. Within these hydrogel-filled conduits, neurites were observed to extend more rapidly than when cultured within the hydrogel alone. The spatial cue also induced a change in tissue architecture, with the cabling of cells within the microconduit. The acceleration of neurite extension was found to be independent of conduit diameter within the range of 200 to 635 microm. Finally, our in vitro model enabled quantification of the effect of combining spatial control and localized nerve growth factor delivery.
Tissue Inhibitor of Matrix Metalloproteinases-1 Loaded Poly(lactic-co-glycolic Acid) Nanoparticles for Delivery Across the Blood-brain Barrier International Journal of Nanomedicine. 2014 | Pubmed ID: 24531257 The aim of this study was to develop poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) for delivery of a protein - tissue inhibitor of matrix metalloproteinases 1 (TIMP-1) - across the blood-brain barrier (BBB) to inhibit deleterious matrix metalloproteinases (MMPs).