Multiplex Immunofluorescence and Spatial Image Analysis of the Tumor Microenvironment

June 2nd, 2023

Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment (Video) | JoVE

01:36 min

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Multiplex Immunofluorescence Staining and Image Analysis (Video) | JoVE

04:44 min

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Nicolas Huyghe¹, Elena Benidovskaya¹, Simon Beyaert¹, Aurélie Daumerie⁴, Finoula Maestre Osorio¹, Frank Aboubakar Nana^2,3, Caroline Bouzin⁴, Marc Van den Eynde^1,5

¹Institut de Recherche Expérimentale et Clinique (IREC), Pôle MIRO, Université Catholique de Louvain (UCLouvain), ²Institut de Recherche Expérimentale et Clinique (IREC), Pôle de Pneumologie, ORL et Dermatologie (PNEU), Université Catholique de Louvain (UCLouvain), ³Division of Pneumology, Cliniques Universitaires St-Luc, Université Catholique de Louvain (UCLouvain), ⁴IREC Imaging Platform, Université Catholique de Louvain (UCLouvain), ⁵Institut Roi Albert II, Department of Medical Oncology and Gastroenterology, Cliniques Universitaires St-Luc

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本稿では、チラミドシグナル増幅(TSA)マルチプレックス免疫蛍光法(mIF)と画像解析および空間解析を組み合わせたプロトコルについて説明します。このプロトコルは、実験室で利用可能なスライドスキャナーに応じて、スライドごとに2〜6個の抗原を染色するために、ホルマリン固定パラフィン包埋(FFPE)セクションで使用できます。

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Multiplex Immunofluorescence and Spatial Image Analysis of the Tumor Microenvironment

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