Multiplex Immunofluorescence and Spatial Image Analysis of the Tumor Microenvironment

June 2nd, 2023

Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment (Video) | JoVE

01:36 min

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Multiplex Immunofluorescence Staining and Image Analysis (Video) | JoVE

04:44 min

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Nicolas Huyghe¹, Elena Benidovskaya¹, Simon Beyaert¹, Aurélie Daumerie⁴, Finoula Maestre Osorio¹, Frank Aboubakar Nana^2,3, Caroline Bouzin⁴, Marc Van den Eynde^1,5

¹Institut de Recherche Expérimentale et Clinique (IREC), Pôle MIRO, Université Catholique de Louvain (UCLouvain), ²Institut de Recherche Expérimentale et Clinique (IREC), Pôle de Pneumologie, ORL et Dermatologie (PNEU), Université Catholique de Louvain (UCLouvain), ³Division of Pneumology, Cliniques Universitaires St-Luc, Université Catholique de Louvain (UCLouvain), ⁴IREC Imaging Platform, Université Catholique de Louvain (UCLouvain), ⁵Institut Roi Albert II, Department of Medical Oncology and Gastroenterology, Cliniques Universitaires St-Luc

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I denna artikel beskrivs ett protokoll för manuell tyramidsignalförstärkning (TSA) multiplex immunofluorescens (mIF) kombinerat med bildanalys och rumslig analys. Detta protokoll kan användas med formalinfixerade paraffininbäddade (FFPE) sektioner för färgning av två till sex antigener per glas beroende på vilken glidskanner som finns i laboratoriet.

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Multiplex Immunofluorescence and Spatial Image Analysis of the Tumor Microenvironment

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