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Epigenetic Inheritance and ChIP in
Caenorhabditis elegans
May 05, 2023
線虫
の世代間エピジェネティック遺伝の蛍光レポーターとクロマチン免疫沈降(ChIP)による解析
01:27 min
•
Caenorhabditis elegans
P0世代の胚のRNAi-NGMプレートへの漂白とプレーティング
02:36 min
•
生殖細胞系GFP発現のための
Caenorhabditis elegans
P0生成の継代とスコアリング
02:02 min
•
クロマチン免疫沈降(ChIP)のためのF1世代
線虫
の収集
00:59 min
•
ChIP用F1世代線
虫
のホルムアルデヒド架橋
01:18 min
•
ホルムアルデヒド架橋線
虫
の超音波処理とクロマチン免疫沈降
02:41 min
•
Chengyin Li*
1
,
Phoebe A. W. Bhagoutie*
1
,
Victor Lao
,
Arneet L. Saltzman
1
Department of Cell and Systems Biology
,
University of Toronto
Summary
Automatic Translation
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このプロトコルはC
.のelegans
のRNAi誘導されたサイレンシングおよび準のchromatinの修正のエピジェネティックな遺伝を調査するRNAの干渉およびChIPの試金を記述する。
Article
