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Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution (Video) | JoVE
Mouse Cardiac Progenitor Cell Isolation, Digestion, and Freezing (Video) | JoVE
Embryonic Heart Cell Thawing and Removal of Dead Cells (Video) | JoVE
Nuclei Isolation from Cardiac Progenitor Cells (Video) | JoVE
在这里,我们提出了一个描述细胞核制备的协议。在将心脏组织进行显微切割和酶解离成单细胞后,冷冻祖细胞,然后分离纯活细胞,用于单核RNA测序和单核测定,用于转座酶可接近染色质和高通量测序分析。
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