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Efficient Rat Bone Marrow Neutrophil Extracellular Trap Isolation

April 26th, 2024
*1, *1, 2,3, 1,4, 1,4, 1,4
1Department of Cardiovascular Surgery, West China Hospital, Sichuan University, 2Department of Integrated Traditional Chinese and Western Medicine, Sichuan Provincial Pancreatitis Centre, West China Hospital, Sichuan University, 3West China-Liverpool Biomedical Research Centre, West China Hospital, Sichuan University, 4Cardiovascular Surgery Research Laboratory, West China Hospital, Sichuan University
* These authors contributed equally

Summary
Automatic Translation

Denne undersøkelsen skisserer to teknikker for å isolere rikelig nøytrofile ekstracellulære feller (NET) fra rottebenmarg. Den ene metoden kombinerer et kommersielt nøytrofilt isolasjonssett med sentrifugering av tetthetsgradient, mens den andre bare benytter sentrifugering av tetthetsgradient. Begge tilnærmingene gir funksjonelle NET som overgår de fra nøytrofiler i perifert blod.

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