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Efficient Rat Bone Marrow Neutrophil Extracellular Trap Isolation

April 26th, 2024
*1, *1, 2,3, 1,4, 1,4, 1,4
1Department of Cardiovascular Surgery, West China Hospital, Sichuan University, 2Department of Integrated Traditional Chinese and Western Medicine, Sichuan Provincial Pancreatitis Centre, West China Hospital, Sichuan University, 3West China-Liverpool Biomedical Research Centre, West China Hospital, Sichuan University, 4Cardiovascular Surgery Research Laboratory, West China Hospital, Sichuan University
* These authors contributed equally

Summary
Automatic Translation

Esta investigación describe dos técnicas para aislar abundantes trampas extracelulares de neutrófilos (TNE) de la médula ósea de rata. Un método combina un kit comercial de aislamiento de neutrófilos con centrifugación en gradiente de densidad, mientras que el otro emplea solo centrifugación en gradiente de densidad. Ambos enfoques producen TNE funcionales que superan a los de los neutrófilos de sangre periférica.

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