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TMEM200A: A Novel Oncogene Regulating EMT and PI3K/AKT in Gastric Cancer

September 15th, 2023

Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies (Video) | JoVE

Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies (Video) | JoVE

01:14 min

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Transfection of TMEM200A siRNA in Stomach Adenocarcinoma Cell Lines (Video) | JoVE

Transfection of TMEM200A siRNA in Stomach Adenocarcinoma Cell Lines (Video) | JoVE

01:21 min

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Quantification of mRNA in TMEM200A siRNA Transfected Stomach Adenocarcinoma Cells (Video) | JoVE

Quantification of mRNA in TMEM200A siRNA Transfected Stomach Adenocarcinoma Cells (Video) | JoVE

01:33 min

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Protein Detection in TMEM200A siRNA Transfected Stomach Adenocarcinoma Cells Using Western Blotting (Video) | JoVE

Protein Detection in TMEM200A siRNA Transfected Stomach Adenocarcinoma Cells Using Western Blotting (Video) | JoVE

02:49 min

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Determining the Cell Viability of TMEM200A siRNA Transfected HGC-27 Cell Lines Using CCK-8 Assays (Video) | JoVE

Determining the Cell Viability of TMEM200A siRNA Transfected HGC-27 Cell Lines Using CCK-8 Assays (Video) | JoVE

00:57 min

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Yaowen Zhang¹, Shanshan Kuang¹, Hancheng Qin², Na Zhao¹, Yixin Yang¹, Jisheng Xie¹

¹Department of Histology and Embryology, Youjiang Medical University for Nationalities, ²Department of Pathophysiology, Youjiang Medical University for Nationalities

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ここでは、複数のバイオインフォマティクスツールを組み合わせて、がんにおける TMEM200A の生物学的機能を研究するプロトコルを紹介します。さらに、バイオインフォマティクスの予測を実験的に検証します。

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