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High-Resolution 3D Reconstruction of Human Brain with SHORT and LSFM

January 26th, 2024
*1, *1, *1,2, 1, 1,3, 1, 1,4,5, 1,4,6, 1,4,5
1European Laboratory for Non-linear Spectroscopy (LENS), University of Florence, 2Division of Physiology, Department of Experimental and Clinical Medicine, University of Florence, 3Department of Physics, University of Pisa, 4National Research Council - National Institute of Optics (CNR-INO), 5Department of Physics and Astronomy, University of Florence, 6Department of Biology, University of Florence
* These authors contributed equally

Summary
Automatic Translation

El presente protocolo proporciona un procedimiento paso a paso para la limpieza óptica rápida y simultánea, el marcaje multirredondo y la reconstrucción volumétrica en 3D de decenas de secciones cerebrales humanas postmortem mediante la combinación de la técnica de transformación tisular corta (S WITCH - H2O2 - Antigen Retrieval - 2,2'-tiodiethanol [TDE]) con imágenes de microscopía de fluorescencia de lámina de luz en un protocolo rutinario de alto rendimiento.

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