Xavier Roucou’s lab, at the Université de Sherbrooke, presents a protocol for using OpenProt databases when interrogating mass spectrometry datasets.
Thomas H. Gillingwater’s lab, at the University of Edinburgh, describes an approach for comparing protein levels across tissues and through development using standardized quantitative western blotting.
Arthur Luhur and colleagues, at Indiana University Bloomington, demonstrate protocols for thawing, subculturing, and cryopreserving Drosophila cell lines.
Erik R. Duboué’s lab, at Florida Atlantic University, presents approaches for the manipulation of genes in the evolutionary model system Astyanax mexicanus.
Jennifer R. Wood’s lab, at the University of Nebraska-Lincoln, demonstrates a protocol for counting the number of mRNAs in individual murine oocytes using single molecule RNA fluorescence in situ hybridization.
Hamed Jafar-Nejad’s lab, at Baylor College of Medicine, describes a method for quantifying the bile duct density in the mouse liver for determining effects of genetic and environmental modifiers.
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This collection focuses on genetics and incorporates five broad subdisciplines: the genetics of individuals and populations; genetics and disease; gene expression; epigenetics; and genetic engineering.
This collection features three model organisms commonly used in life sciences research, and also covers methodologies to maintain them in the laboratory.
This collection introduces the field of developmental biology and covers five areas, including developmental genetics and stem cell biology.
This collection provides both general and specific safety guidelines to be followed when working with hazardous materials and equipment.