JoVE Newsletter – November 2018

Indrani Mukherjee, Ph.D.
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Force-Sensitive Protein Dynamics in Living Cells

Dr. Brenton Hoffman’s lab, at Duke University, presents a protocol for the simultaneous use of Förster resonance energy transfer-based tension sensors to measure protein load and fluorescence recovery after photobleaching.

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Lymphocyte Isolation from Murine Peyer’s Patches

Dr. Vassiliki Boussiotis’ lab, at Harvard Medical School, presents a novel and effective protocol for the isolation of lymphocytes from Peyer’s Patches.

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Probing Cell Mechanics in the Drosophila Embryo

Dr. Pierre-François Lenne’s lab, at Aix-Marseille Université, presents a setup of optical tweezers coupled to a light sheet microscope, and its implementation to probe cell mechanics without beads in the Drosophila embryo.

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Antigen-specific T Cells with Magnetic Nanoparticles

Dr. Jonathan Schneck’s lab, at Johns Hopkins University, provides a protocol to develop a magnetic particle which can bind to antigen-specific T cells to enrich these cells.

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Organic Particulate Matter in the Harvard Environmental Chamber

Dr. Scot Martin’s lab, at Harvard University, describes operation procedures for the Harvard Environmental Chamber and related instrumentation for measuring gaseous and particle species.

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3D Printing of a Complex Aortic Anomaly

Dr. Chunsheng Wang’s lab, at Fudan University, presents a protocol to use 3D printed models for pre-operative planning and intra-operative reorganization of complicated vascular locations.

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New Techniques and Approaches for the Assessment or Intervention in Learning Disabilities and ADHD

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Minimally Invasive Pancreatic Surgery

Prostate Organoid Research Techniques and Methods: Three-dimensional (3D) Culture, Spin-down, Dimpled Parafilm, and Fusion Method

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RNA/DNA In Situ Hybridization techniques

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Nursing Skills

This Sci Ed collection demonstrates medication preparation and administration.

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Neuropsychology

This Sci Ed collection presents multidisciplinary techniques in behavior, neurophysiology, anatomy, and functional imaging.

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Electrical Engineering

This Sci Ed collection starts with an electrical safety video that introduces the best practices for commonly used equipment in an electrical laboratory.

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Genetics

This Sci Ed collection focuses on genetics and incorporates five broad subdisciplines.

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