发育生物学，第108，基因编辑，干细胞，CRISPR / Cas9，ZFN，TALEN，AAVS1，电，hPSCs Protocols and Video Articles

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Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor

Authors

Author

Trevor Cerbini¹, Yongquan Luo¹, Mahendra S. Rao², Jizhong Zou¹

Institution

¹National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, ²Q Therapeutics

Journal (Developmental Biology)

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

Authors

Author

Linyuan Ma*¹, Lydia Jang*¹, Jian Chen², Jun Song¹, Dongshan Yang¹, Jifeng Zhang¹, Y. Eugene Chen¹, Jie Xu¹

Institution

¹Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical Center, ²Celetrix LLC

Journal (Genetics)

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Authors

Author

Daniel E. Bauer*^1,2,3, Matthew C. Canver*¹, Stuart H. Orkin^1,2,3,4

Institution

¹Harvard Medical School, ²Division of Hematology/Oncology, Boston Children's Hospital, ³Department of Pediatric Oncology, Dana-Farber Cancer Institute, ⁴Howard Hughes Medical Institute

Journal (Biology)

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

Authors

Author

Kyle E. Simpson¹, Shoaib Faizi¹, Ravichandra Venkateshappa¹, Mandy Yip¹, Raj Johal¹, Damon Poburko¹, Yen May Cheng¹, Diana Hunter¹, Eric Lin¹, Glen F. Tibbits¹, Thomas W. Claydon¹

Institution

¹Department of Biomedical Physiology and Kinesiology, Simon Fraser University

Journal (Biology)

| Methods Collections

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System

Authors

Author

Shenglan Li*^1,2, Haipeng Xue*^1,2, Bo Long^1,2,5, Li Sun^1,2,6, Tai Truong^1,2,4,7, Ying Liu^1,2,3

Institution

¹Department of Neurosurgery, The University of Texas Health Science Center at Houston, ²Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center at Houston, ³The Senator Lloyd & B. A. Bentsen Center for Stroke Research, Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center at Houston, ⁴Summer Research Program, Office of Educational Programs, The University of Texas Health Science Center at Houston, ⁵Department of Anesthesiology, Shengjing Hospital, China Medical University, ⁶Department of Oncology, Renji Hospital, Shanghai Jiaotong University School of Medicine, ⁷Biology Department, University of West Georgia

Journal (Developmental Biology)

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Authors

Author

Andi M. Wilson^1,2, Brenda D. Wingfield^1,2

Institution

¹Department of Biochemistry, Genetics & Microbiology, University of Pretoria, ²Forestry & Agricultural Biotechnology Institute (FABI), University of Pretoria

Journal (Genetics)

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Education

Showing 1 - 6 of 1,632 results for "发育生物学，第108，基因编辑，干细胞，CRISPR / Cas9，ZFN，TALEN，AAVS1，电，hPSCs"

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CRISPR

JoVE Core (Core: 生物)

CRISPR

JoVE Core (Core: 分子生物学)

CRISPR and crRNAs

JoVE Core (Core: 分子生物学)

Genome Editing

Science Education (Advanced Biology)

iPS Cell Differentiation

JoVE Core (Core: Cell Biology)

Lineage Commitment

JoVE Core (Core: Cell Biology)

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