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Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
 

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Article DOI: 10.3791/3715-v 08:06 min December 12th, 2011
December 12th, 2011

章节

总结概括

Using Luminex Corporation’s xMAP microsphere technology, we have developed the Multiplexed Fluorometric ImmunoAssay (MFIA) for serosurveillance of various laboratory animal species. The MFIA is a suspension microarray where antigen, tissue control or immunoglobulins are covalently linked to color-coded polystyrene microspheres. The MFIA testing method as well as various troubleshooting topics is addressed.

Tags

Multiplexed Fluorometric ImmunoAssay Diagnostic Testing Animal Models Biomedical Research Infectious Agents Viruses Mycoplasma Microorganisms Serology Antibody Responses Serosurveillance Enzyme-linked Immunosorbent Assay (ELISA) Multiplexed Assay Microspheres Color Sets Serum Reagents Disposables Internal Control Beads Tissue Control IgG Anti-test Serum Species Immunoglobulin (αIg)
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