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Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples
JoVE 杂志
医学
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JoVE 杂志 医学
Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples

Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples

DOI:
10.3791/51116-v

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14:39 min

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April 21, 2014

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, , , , , , ,
1Department NeuroFarBa, Division of Pharmacology,University of Florence, 2Department of Clinical and Experimental Medicine, Division of Physiology,University of Florence

Chapters

  • 00:05Title
  • 02:47Collection and Processing of Myocardial Samples
  • 03:52Washing and Digestion of Myocardial Chunks
  • 06:43Cell Resuspension and Ca2+ Readapation
  • 08:28Functional Evaluation of Isolated Cardiomyocytes
  • 11:11Results
  • 13:16Conclusion

Summary

自动翻译

自动翻译

Current knowledge on the cellular basis of cardiac diseases mostly relies on studies on animal models. Here we describe and validate a novel method to obtain single viable cardiomyocytes from small surgical samples of human ventricular myocardium. Human ventricular myocytes can be used for electrophysiological studies and drug testing.

Tags

CardiomyocytesVentricular MyocardiumCardiac SurgeryElectrophysiologyIntracellular CalciumCardiac DiseaseCellular And Molecular BasisTherapeutic TargetsTranslational Value

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