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Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli
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Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli

Article DOI: 10.3791/52850-v • 12:19 min • June 27th, 2015
June 27th, 2015 •
1, 1, 1, 1
1Department of Physiology, Medical College of Wisconsin

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Changes in the intracellular calcium levels in the podocytes are one of the most important means to control the filtration function of glomeruli. Here we explain a high-throughput approach that allows detection of real-time calcium handling and single ion channels activity in the podocytes of the freshly isolated glomeruli.

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Single-channel Analysis Calcium Imaging Podocytes Glomeruli Renal Diseases Proteinuria Foot Process Effacement Intracellular Calcium Concentration Calcium-conducting Ion Channels Calcium Handling Pathological States Cultured Cells Transfected Cells Freshly Isolated Glomerulus Glomerular Filtration Apparatus Real-time Detection
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