-1::1
Simple Hit Counter
Skip to content

Products

Solutions

×
×
Sign In

CN

EN - EnglishCN - 简体中文DE - DeutschES - EspañolKR - 한국어IT - ItalianoFR - FrançaisPT - Português do BrasilPL - PolskiHE - עִבְרִיתRU - РусскийJA - 日本語TR - TürkçeAR - العربية
Sign In Start Free Trial

RESEARCH

JoVE Journal

Peer reviewed scientific video journal

Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
View All
JoVE Encyclopedia of Experiments

Video encyclopedia of advanced research methods

Biological Techniques
Biology
Cancer Research
Immunology
Neuroscience
Microbiology
JoVE Visualize

Visualizing science through experiment videos

EDUCATION

JoVE Core

Video textbooks for undergraduate courses

Analytical Chemistry
Anatomy and Physiology
Biology
Calculus
Cell Biology
Chemistry
Civil Engineering
Electrical Engineering
View All
JoVE Science Education

Visual demonstrations of key scientific experiments

Advanced Biology
Basic Biology
Chemistry
View All
JoVE Lab Manual

Videos of experiments for undergraduate lab courses

Biology
Chemistry

BUSINESS

JoVE Business

Video textbooks for business education

Accounting
Finance
Macroeconomics
Marketing
Microeconomics

OTHERS

JoVE Quiz

Interactive video based quizzes for formative assessments

Authors

Teaching Faculty

Librarians

K12 Schools

Biopharma

Products

RESEARCH

JoVE Journal

Peer reviewed scientific video journal

JoVE Encyclopedia of Experiments

Video encyclopedia of advanced research methods

JoVE Visualize

Visualizing science through experiment videos

EDUCATION

JoVE Core

Video textbooks for undergraduates

JoVE Science Education

Visual demonstrations of key scientific experiments

JoVE Lab Manual

Videos of experiments for undergraduate lab courses

BUSINESS

JoVE Business

Video textbooks for business education

OTHERS

JoVE Quiz

Interactive video based quizzes for formative assessments

Solutions

Authors
Teaching Faculty
Librarians
K12 Schools
Biopharma

Language

zh_CN

EN

English

CN

简体中文

DE

Deutsch

ES

Español

KR

한국어

IT

Italiano

FR

Français

PT

Português do Brasil

PL

Polski

HE

עִבְרִית

RU

Русский

JA

日本語

TR

Türkçe

AR

العربية

    Menu

    JoVE Journal

    Behavior

    Biochemistry

    Bioengineering

    Biology

    Cancer Research

    Chemistry

    Developmental Biology

    Engineering

    Environment

    Genetics

    Immunology and Infection

    Medicine

    Neuroscience

    Menu

    JoVE Encyclopedia of Experiments

    Biological Techniques

    Biology

    Cancer Research

    Immunology

    Neuroscience

    Microbiology

    Menu

    JoVE Core

    Analytical Chemistry

    Anatomy and Physiology

    Biology

    Calculus

    Cell Biology

    Chemistry

    Civil Engineering

    Electrical Engineering

    Introduction to Psychology

    Mechanical Engineering

    Medical-Surgical Nursing

    View All

    Menu

    JoVE Science Education

    Advanced Biology

    Basic Biology

    Chemistry

    Clinical Skills

    Engineering

    Environmental Sciences

    Physics

    Psychology

    View All

    Menu

    JoVE Lab Manual

    Biology

    Chemistry

    Menu

    JoVE Business

    Accounting

    Finance

    Macroeconomics

    Marketing

    Microeconomics

Start Free Trial
Loading...
Home
JoVE Journal
Chemistry
调优并行分段流动列和启用多重检测
调优并行分段流动列和启用多重检测
JoVE Journal
Chemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Chemistry
Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection

调优并行分段流动列和启用多重检测

Full Text
7,974 Views
08:01 min
December 15, 2015

DOI: 10.3791/53448-v

Sercan Pravadali-Cekic1, Danijela Kocic1, Stanley Hua1, Andrew Jones1, Gary R. Dennis1, R. Andrew Shalliker1

1School of Science and Health,University of Western Sydney

AI Banner

Please note that some of the translations on this page are AI generated. Click here for the English version.

Overview

This article presents a protocol for tuning parallel segmented flow chromatography columns, facilitating multiplex detection. The method is applicable in various fields, including health, environmental sciences, and forensics.

Key Study Components

Area of Science

  • Chromatography
  • Analytical Chemistry
  • Complex Sample Analysis

Background

  • Parallel segmented flow columns enhance detection capabilities.
  • This method addresses complex sample analysis challenges.
  • It is relevant for industries like food and beverage.
  • Speedy analysis is a significant advantage of this technique.

Purpose of Study

  • To enable multiplex detection in complex samples.
  • To develop strategies for chemical fingerprinting.
  • To differentiate between authentic products and counterfeits.

Methods Used

  • High-Performance Liquid Chromatography (HPLC)
  • Parallel segmented flow column operation
  • Multiplex detection protocols
  • Sample analysis of complex mixtures

Main Results

  • Successful tuning of chromatography columns for multiplex detection.
  • Comprehensive analysis of complex samples achieved.
  • Identification of chemical and bioactive properties in mixtures.
  • Enhanced speed of analysis compared to traditional methods.

Conclusions

  • The method provides a robust approach for analyzing complex samples.
  • It supports the differentiation of products in competitive markets.
  • This technique can be applied across various scientific disciplines.

Frequently Asked Questions

What is the main advantage of using parallel segmented flow chromatography?
It allows for multiplex detection, enhancing the analysis of complex samples.
In which fields can this method be applied?
It is applicable in health, environmental sciences, forensics, and food analysis.
How does this method improve sample analysis speed?
The multiplex detection protocols enable quicker analysis compared to traditional methods.
What types of samples can be analyzed using this technique?
Complex mixtures such as coffee, wine, and other bioactive substances.
What is the significance of chemical fingerprinting?
It helps manufacturers distinguish their products from counterfeit varieties.
Is this method suitable for routine analysis?
Yes, it is designed for comprehensive and efficient sample analysis.

在这里,我们提出了一种作和调整平行分段流式色谱柱以实现多重检测的方案。

该 HPLC 程序的总体目标是调整平行分段流柱以实现多重检测。该方法回答了健康和福祉、环境科学、法医学以及任何需要分析复杂样品(如生物发现)的领域的关键问题。该技术可以进行全面的样品分析,同时还可以获得有关复杂混合物中物质的化学和生物活性性质的信息。

多重检测方案还允许快速分析。这种方法有助于开发定义复杂混合物(如咖啡和葡萄酒)的化学指纹的策略,使制造商能够区分他们的产品和廉价的假冒品种。最初,不将整个样品发送到一个检测器进行准确分析可能会让人感觉不合常规。

View the full transcript and gain access to thousands of scientific videos

View the full transcript and gain access to thousands of scientific videos

Sign In Start Free Trial

Explore More Videos

化学 第106 并行分段流动 多重检测 柱技术 高效液相色谱法

Related Videos

使用介电泳分离珠和多通道微流控芯片细胞和层流

09:45

使用介电泳分离珠和多通道微流控芯片细胞和层流

Related Videos

28.1K Views

同时捕获实时图像在两个发射通道采用了双摄像头发射分光系统:应用到细胞粘附

10:30

同时捕获实时图像在两个发射通道采用了双摄像头发射分光系统:应用到细胞粘附

Related Videos

10K Views

柱后衍生用反应流高效液相色谱柱

06:25

柱后衍生用反应流高效液相色谱柱

Related Videos

16K Views

幕式柱:效率和灵敏度的优化

06:44

幕式柱:效率和灵敏度的优化

Related Videos

7K Views

使用多色荧光检测液滴微流控光纤

10:21

使用多色荧光检测液滴微流控光纤

Related Videos

11.4K Views

与复用电子检测的粒子的空间跟踪微流体平台

11:54

与复用电子检测的粒子的空间跟踪微流体平台

Related Videos

9.9K Views

微流控成像流式细胞仪通过不对称检测时间延伸光学显微镜(ATOM)

07:19

微流控成像流式细胞仪通过不对称检测时间延伸光学显微镜(ATOM)

Related Videos

10.8K Views

基于复用等温放大诊断平台检测 Zika、基孔肯亚和登革热1

06:18

基于复用等温放大诊断平台检测 Zika、基孔肯亚和登革热1

Related Videos

15K Views

多重条形码图像分析,用于石蜡组织样品单细胞分析中的免疫分析和空间映射表征

08:18

多重条形码图像分析,用于石蜡组织样品单细胞分析中的免疫分析和空间映射表征

Related Videos

2.3K Views

在多重血清学检测中同时检测不同抗体类别

05:25

在多重血清学检测中同时检测不同抗体类别

Related Videos

2K Views

JoVE logo
Contact Us Recommend to Library
Research
  • JoVE Journal
  • JoVE Encyclopedia of Experiments
  • JoVE Visualize
Business
  • JoVE Business
Education
  • JoVE Core
  • JoVE Science Education
  • JoVE Lab Manual
  • JoVE Quizzes
Solutions
  • Authors
  • Teaching Faculty
  • Librarians
  • K12 Schools
  • Biopharma
About JoVE
  • Overview
  • Leadership
Others
  • JoVE Newsletters
  • JoVE Help Center
  • Blogs
  • JoVE Newsroom
  • Site Maps
Contact Us Recommend to Library
JoVE logo

Copyright © 2026 MyJoVE Corporation. All rights reserved

Privacy Terms of Use Policies
WeChat QR code