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Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method
JoVE 杂志
生物学
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JoVE 杂志 生物学
Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method
DOI:

07:56 min

August 06, 2016

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Chapters

  • 00:05Title
  • 01:08Preparation of FINA Sample Preparation Module
  • 02:27Preparation of qPCR Tube
  • 03:12Preparation of Contrived Specimen
  • 04:00FINA Extraction
  • 05:57Results: FINA Enables Efficient Detection of Proviral HIV DNA from Whole Blood
  • 07:20Conclusion

Summary

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We describe here a simple and rapid paper-based DNA extraction method of HIV proviral DNA from whole blood detected by quantitative PCR. This protocol can be extended for use in detecting other genetic markers or using alternative amplification methods.

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