Journal
/
生物学
/
Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples
JoVE 杂志
生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物学
Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples

Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples

DOI:
10.3791/55031-v

•

10:57 min

•

February 03, 2017

•

, , , ,
1Biodiversity and Conservation Department,Real Jardín Botánico, CSIC, 2Research Support Unit,Real Jardín Botánico, CSIC, 3Mycology Department,Real Jardín Botánico, CSIC, 4Division of Glycoscience, AlbaNova University Center,Royal Institute of Technology (KTH)

Chapters

  • 00:05Title
  • 00:57Collection and Fixation of Developing and Fully Formed Plant Structures
  • 02:04Dissection and Dehydration of Developing and Fully Formed Plant Structures
  • 03:02Mounting and Preparing Plant Tissues for SEM Observation of Developing and Fully Formed Plant Structures
  • 04:20Study of Cyst Behavior of Saprolegnia (Oomycetes) on Different Surfaces
  • 06:04Study of Herbarium Fungal Spores of Phellorinia herculanea under SEM
  • 07:20Results: Recovery of Plant Development, Differential Spine Growth of Saprolegnia parasitica, and Nurse Cells in Spores of Phellorinia herculanea f. stellata
  • 09:01Conclusion

Summary

自动翻译

自动翻译

Problems in the processing of biological samples for scanning electron microscopy observation include cell collapse, treatment of samples from wet microenvironments and cell destruction. Low-cost and relatively rapid protocols suited for preparing challenging samples such as floral meristems, oomycete cysts, and fungi (Agaricales) are compiled and detailed here.

Tags

Scanning Electron Microscopy (SEM)SEM ProtocolsPlant SamplesOomycete SamplesFungal SamplesFixationFAA Fixative70% EthanolFormaldehydeAcetic AcidDehydrationCritical Point DryingSample MountingSEM Sample Holders

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

Analysis of Autophagy in Penicillium chrysogenum by Using Starvation Pads in Combination With Fluorescence Microscopy

A Standardized Method for the Analysis of Liver Sinusoidal Endothelial Cells and Their Fenestrations by Scanning Electron Microscopy

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix

Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy (SEM)

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples

Read Article