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A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level

Article DOI: 10.3791/55106-v • 11:14 min • January 10th, 2017
January 10th, 2017 •
1, 2, 1, 1, 1
1Mechanical Engineering and Materials Science, Duke University, 2Huacells Corp

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A microfluidic chip was fabricated to produce pairs of gold dots for tandem bubble generation and fibronectin-coated islands for single-cell patterning nearby. The resultant flow field was characterized by particle image velocimetry and was employed to study various bioeffects, including cell membrane poration, membrane deformation, and intracellular calcium response.

Tags

Microfluidic System Surface Patterning Cavitation Bubble Cell Interaction Bioeffects Single-cell Level Experimental Procedure Tandem Bubbles Target Cells Precision Control Therapeutic Applications Sound Operation Microfabrication Procedures Clean Room Gold Dot Laser Energy Spin Coating
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