通过两个互补细胞同步方案研究细胞周期调控的基因表达
27,161 Views
•
12:02 min
•
June 06, 2017
DOI:
成績單
Automatically generated
Summary
Automatically generated
我们报告两个细胞同步协议，提供一个上下文，用于研究与细胞周期的特定阶段相关的事件。我们表明，这种方法可用于分析未受扰动细胞周期中特定基因的调节，或暴露于影响细胞周期的试剂。
Related Videos
06:53
Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
Related Videos
8182 Views
10:23
Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations
Related Videos
10894 Views
12:19
Analysis of Cell Cycle Position in Mammalian Cells
Related Videos
60029 Views
11:23
Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry
Related Videos
20987 Views
08:02
Synchronization of Caulobacter Crescentus for Investigation of the Bacterial Cell Cycle
Related Videos
11815 Views
08:52
Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization
Related Videos
19109 Views
08:33
Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
Related Videos
14197 Views
07:59
Alignment of Synchronized Time-Series Data Using the Characterizing Loss of Cell Cycle Synchrony Model for Cross-Experiment Comparisons
Related Videos
1267 Views
12:02
Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
Related Videos
27.1K Views
08:02
Synchronization of Caulobacter Crescentus for Investigation of the Bacterial Cell Cycle
Related Videos
11.8K Views
Read Article
Cite this Article
Apraiz, A., Mitxelena, J., Zubiaga, A. Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols. J. Vis. Exp. (124), e55745, doi:10.3791/55745 (2017).
Copy