斑马斑马模型系统中 CRISPR/Cas9-generated 基因挖空的高效生产与鉴定
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11:27 min
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August 28, 2018
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Summary
Automatically generated
斑马斑马(斑马鱼) 在模型系统中的靶向基因组编辑由于 CRISPR 方法的出现而大大促进。在这里, 我们描述了一个简化的, 健壮的协议, 以生成和鉴定 CRISPR 衍生的无意义的等位基因, 包括 heteroduplex 移动性检测和识别突变使用下一代测序。
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Cite this Article
Sorlien, E. L., Witucki, M. A., Ogas, J. Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio. J. Vis. Exp. (138), e56969, doi:10.3791/56969 (2018).
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