Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice

Meng Wang; Yuanyuan Huang; Wangpeng Gu; Haikun Wang

doi:10.3791/60523

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice

小鼠感染甲型流感病毒期间T卵泡帮助细胞和胚芽中心反应的评价

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07:07 min

June 27, 2020

DOI: 10.3791/60523-v

Meng Wang*¹, Yuanyuan Huang*¹, Wangpeng Gu^2,3, Haikun Wang¹

¹CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences,University of Chinese Academy of Sciences, ²School of Life Science,University of Science and Technology of China, ³State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences,University of Chinese Academy of Sciences

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本文描述了在流感病毒感染小鼠模型中评估Tfh和GC B反应的协议。

滤泡辅助性 T 细胞是新发现的 CD4 T 细胞亚群，可在高亲和力动物生产中为 B 细胞提供帮助。证明流感病毒感染中 Tfh 细胞的鉴定将有助于新研究人员了解如何执行 Tfh 相关研究的必要技术。由于不适当的病毒滴度会导致死亡或免疫反应过弱，因此我们建议在进行实验之前确定滴度。

用于 8 周龄雄性 C57 黑色 6 只小鼠的 PR8 病毒接种。在生物安全 2 级条件下，解冻冰上储存的零下 80 摄氏度的 PR8 病毒原液。涡旋病毒以获得均匀的溶液。

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本月在JoVE 第160期 T卵泡辅助细胞细菌中心甲型流感病毒感染 Bcl6 四体流动细胞切除术酶链接免疫细胞检测免疫荧光