小鼠模型在转变过程中的应用 肺炎链球菌 从定植者到病原体：病毒共感染重现年龄加剧的疾病进程
4.3K 次观看
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被引用 4 次
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12:21 分钟
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2022年9月28日
DOI :10.3791/64419-v
2022年9月28日
•4.3K 次观看
Alexsandra Lenhard*1 , Basma H. Joma*2,3 , Nalat Siwapornchai2 , Anders P. Hakansson4 , John M. Leong2,5 , Elsa N. Bou Ghanem1
1Department of Microbiology and Immunology, University at Buffalo School of Medicine, 2Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 3Graduate Program in Immunology, Tufts Graduate School of Biomedical Sciences, 4Department of Translational Medicine, Lund University, 5Stuart B. Levy Center for the Integrated Management of Antimicrobial Resistance, Tufts University
* These authors contributed equally
本文描述了一种新型小鼠模型，用于研究肺炎链球菌在病毒感染期间从无症状定植者转变为致病性病原体的过程。该模型可轻松适用于研究疾病进展不同阶段以及多种宿主中多微生物与宿主-病原体之间的相互作用。
Chapters in this video
0:05
Introduction
0:55
Growing Streptococcus pneumoniae Biofilm
4:15
Intranasal Inoculation of Mice with Biofilm-Grown S. pneumoniae
5:11
Viral Infection with Influenza A Virus (IAV)
6:01
Processing of Infected Tissues for Bacterial Enumeration
8:35
Processing of the Lung Samples for Flow Cytometry
9:07
Results: Enhancement of S. pneumoniae Pathogenicity upon Viral Infection and Advanced Age
11:43
Conclusion
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