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免疫与感染

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使用流细胞测定测量红细胞补充受体1

Measuring Erythrocyte Complement Receptor 1 Using Flow Cytometry

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Measuring Erythrocyte Complement Receptor 1 Using Flow Cytometry

使用流细胞测定测量红细胞补充受体1

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7,153 Views

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07:20 min

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May 19, 2020

DOI:

10.3791/60810-v

DOI:

10.3791/60810-v

•

07:20 min

May 19, 2020

•

7149 Views

Aymric Kisserli^1,2, Sandra Audonnet³, Valérie Duret^2,4, Thierry Tabary^2,4, Jacques Henri Max Cohen², Rachid Mahmoudi^5,6

¹Oncogeriatric Coordination Unit,Reims University Hospitals, Maison Blanche Hospital ²Faculty of Medicine,University of Reims Champagne-Ardenne ³URCACyt, Flow cytometry technical platform,University of Reims Champagne-Ardenne ⁴Department of Immunology,Reims University Hospitals, Robert Debre Hospital ⁵Department of Internal Medicine and Geriatrics,Reims University Hospitals, Maison Blanche Hospital ⁶Faculty of Medicine,University of Reims Champagne-Ardenne

Summary

该方法的目的是通过与已知红细胞CR1密度的三个受试者进行比较，确定任何受试者红细胞CR1密度的CR1密度。该方法使用抗CR1单克隆抗体在受试者红细胞免疫染色后使用流细胞学，并结合使用植物素（PE）的放大系统。

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