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Immunology and Infection

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JoVE Journal Immunology and Infection
Identificatie en karakterisering van immunogene RNA-soorten bij HDM-allergenen die eosinofiele longontsteking moduleren
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Identificatie en karakterisering van immunogene RNA-soorten bij HDM-allergenen die eosinofiele longontsteking moduleren

Article DOI: 10.3791/61183-v • 08:44 min • May 30th, 2020
May 30th, 2020 •
1,2, 1,3, 1
1Department of Microbiology, Immunology and Molecular Genetics, Long School of Medicine, University of Texas Health San Antonio, 2Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, 3Department of Otolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University

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Milieuallergenen zoals huisstofmijt (HDM) bevatten vaak microbiële stoffen die aangeboren immuunreacties activeren om allergische ontstekingen te reguleren. Het hier gepresenteerde protocol toont de identificatie aan van dsRNA-soorten in HDM-allergenen en karakterisering van hun immunogene activiteiten bij het moduleren van eosinofiele longontsteking.

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Immunologie en infectie Probleem 159 BAL bronchoalveolaire lavage dsRNA FACS fluorescentie-geactiveerde celsorling HDM huisstofmijt ISG's interferongeïntimuleerde genen PAMP pathogen-geassocieerd moleculair patroon RT-qPCR
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