利用转录组学分析绘制无序致癌转录因子的结构-功能关系
4.1K 次观看
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被引用 30 次
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09:58 分钟
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2020年6月27日
DOI :10.3791/61564-v
2020年6月27日
•4.1K 次观看
Iftekhar A. Showpnil1,2 , Kyle R. Miller1 , Cenny Taslim1 , Kathleen I. Pishas1 , Stephen L. Lessnick1,3 , Emily R. Theisen1,4
1Center for Childhood Cancer and Blood Diseases, Abigail Wexner Research Institute at Nationwide Children's Hospital, 2Molecular, Cellular, and Developmental Biology Program, The Ohio State University, 3Division of Pediatric Hematology/Oncology/Blood & Marrow Transplant, The Ohio State University, 4Department of Pediatrics, The Ohio State University
内在无序结构域对致癌融合转录因子的功能具有重要作用。为了靶向治疗这些蛋白，需要更深入地理解这些结构域所采用的调控机制。本文中，我们利用转录组学方法，绘制尤因肉瘤中内在无序的 EWS 结构域的重要结构特征。
Chapters in this video
0:04
Introduction
0:55
In Vitro Construct Panel Setup
2:07
Cell Collection
2:47
Construct Expression Validation and Correlative Phenotypic Assay Setup
3:50
Alignment and Transcript Counting Pipeline
5:32
Differential Expression
7:40
Results: Representative Disordered Oncogenic Transcription Factor Structure-Function Relationship Mapping
9:31
Conclusion
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