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Chapters
- 00:05Introduction
- 00:52PCR Optimization
- 01:57Parallel Capillary Electrophoresis and Calibration
- 03:18Results: Mutant Allele Expression
- 04:21Conclusion
We developed a cost-effective method to follow non-single nucleotide polymorphism allele dynamics that can easily be adapted to experimental evolution frozen archives. A triplet PCR technique was coupled with automated parallel capillary electrophoresis to quantify the relative frequency of an insertion allele over the course of experimental evolution.
