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JoVE Journal
Bioengineering
斑马鱼心脏收缩的 4D 光片成像
斑马鱼心脏收缩的 4D 光片成像
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
4D Light-sheet Imaging of Zebrafish Cardiac Contraction

斑马鱼心脏收缩的 4D 光片成像

Full Text
1,818 Views
07:07 min
January 5, 2024

DOI: 10.3791/66263-v

Xinyuan Zhang1, Alireza Saberigarakani1, Milad Almasian1, Sohail Hassan1, Manasa Nekkanti1, Yichen Ding1,2,3

1Department of Bioengineering,The University of Texas at Dallas, 2Center for Imaging and Surgical Innovation,The University of Texas at Dallas, 3Hamon Center for Regenerative Science and Medicine,UT Southwestern Medical Center

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Please note that some of the translations on this page are AI generated. Click here for the English version.

该协议利用光片成像来研究斑马鱼幼虫的心脏收缩功能,并通过细胞跟踪和交互式分析深入了解心脏力学。

我们的研究使用4D光片成像来探索斑马鱼的心脏收缩功能,旨在了解单细胞水平的心脏收缩动力学。最重要的实验挑战之一是在观察自然心律的同时实现高空间终端分辨率、心脏功能的动态成像。我们的研究填补了在单细胞分辨率下分析复杂 4D 心脏动力学的空白。

我们利用基于 GPU 的并行计算、虚拟现实和先进的细胞跟踪来解释光片成像数据。我们新颖的虚拟现实平台提供了一种交互式方法来评估区域心脏收缩,为更深入的分析提供操作功能。通过促进用户导向的心脏收缩分析,我们的方案可以增强对心脏发育和疾病的理解。

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