一种高效提取人输卵管上皮细胞用于单细胞分析的方法
1.4K 次观看
•
07:03 分钟
•
2025年3月28日
DOI :10.3791/67753-v
2025年3月28日
•1.4K 次观看
Lauryn Ruegg1,2 , Laura B. James-Allan1,2 , Gabriella DiBernardo1,2 , Christopher Ochoa1,2,3 , Yan-Ruide Li4,5 , Lili Yang2,3,4,5,6,7 , Sanaz Memarzadeh1,2,3,7,8,9
1Department of Obstetrics and Gynecology, David Geffen School of Medicine, University of California, Los Angeles, 2Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, 3Molecular Biology Institute, University of California, Los Angeles, 4Department of Bioengineering, University of California, Los Angeles, 5Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, 6Parker Institute for Cancer Immunotherapy, University of California, Los Angeles, 7The Jonsson Comprehensive Cancer Center, University of California Los Angeles, 8The VA Greater Los Angeles Healthcare System, 9Department of Medical and Molecular Pharmacology, David Geffen School of Medicine, University of California, Los Angeles
本方案描述了一种从人类输卵管上皮组织中分离上皮细胞的一日法。分离得到的上皮细胞可用于二维（2D）培养，或解离为单细胞悬液，进而应用于后续实验，包括流式细胞术和单细胞RNA测序。
Chapters in this video
0:00
Introduction
1:11
Fallopian Tube Collection and Preparation for Epithelial Cell Isolation
2:06
Epithelial Cell Isolation and Dissociation
4:18
Digestion of Isolated Epithelial Cells to Obtain a Single-Cell Suspension
6:02
Representative Results
Related Videos
版权所有 © 2026 MyJoVE Corporation。保留所有权利。