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Chapters
- 00:05Title
- 02:47Preparation of Aggresome Donor Cells: Transfection of mRFP- or eGFP-tagged Human Full-length DISC1
- 03:50Aggresome Purification from Transfected Donor Cells
- 06:43Treatment of Recipient SH-SYBY Cells with mRFP-DISC1 Aggresomes
- 08:13Labeling of Recombinant DISC1(598-785) with DyLight594
- 10:44Treatment of Recipient SH-SY5Y Cells with Labeled Recombinant DISC1(598-785) Protein
- 12:00Representative Invasion Assay Results
- 13:30Conclusion
The generation, purification and cell invasion of intracellular, cytoplasmic full length DISC1 protein aggresomes from cell cultures and of a labeled, multimeric recombinant DISC1 protein fragment in E. coli are described. Cell invasiveness is shown for recipient cells in cell culture and for neurons in vivo after stereotactical brain inoculation.
Tags
Protein AggregationChronic Degenerative Brain ConditionsNeurodegenerative DiseasesAlzheimer’s DiseaseTauParkinson’s DiseaseAlpha-synucleinHuntington’s DiseasePolyglutamineHuntingtinProteostasisMisfolded ProteinsSporadic FormsFamilial FormsSchizophreniaCognitive DeficitDisrupted-in-schizophrenia 1 (DISC1)Scottish FamilyChronic Mental DiseaseInsoluble AggregatesSMRI CCSarkosyl-insoluble DISC1 ImmunoreactivityBiochemical FractionationDisease-associated Polymorphism S704CDISC1 Aggresomes
