Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Staining of Proteins in Gels with Coomassie G-250 …
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
Linking Predation Risk, Herbivore Physiological Stress and Microbial Decomposition of Plant Litter
Journal (Biology)
Linking Predation Risk, Herbivore Physiological Stress and Microbial Decomposition of Plant Litter
A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
Journal (Biology)
A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
 

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

Article DOI: 10.3791/1350 • 07:47 min • August 14th, 2009
August 14th, 2009 •
1, 1
1Protein Expression and Purification Core Facility, EMBL Heidelberg

Chapters

Summary
Automatic Translation

A short protocol for protein staining with Coomassie Brilliant Blue (CBB) G-250 in polyacrylamide gels is described without using organic solvents or acetic acid as in the classical staining procedures with CBB.

Tags

Proteins Gels Coomassie G-250 Staining Organic Solvent Acetic Acid SDS-PAGE Microwave Oven Toxic Solvents Safety Considerations Alternative Composition Bidistilled Water HCl Washing Heating Gel Protein Staining
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter