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Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation

Article DOI: 10.3791/50648-v • 09:48 min • October 9th, 2013
October 9th, 2013 •
1,2, 3, 1, 1,4,5, 3
1Department of Cell and Developmental Biology, University of Pennsylvania, 2Biomedical Graduate Studies, University of Pennsylvania, 3Department of Animal Biology and Center for Animal Transgenesis and Germ Cell Research, University of Pennsylvania, 4Department of Genetics, University of Pennsylvania, 5Department of Biology, University of Pennsylvania

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Summary
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The STA-PUT method allows for the separation of different populations of spermatogenic cells based on size and density.

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Separation Of Spermatogenic Cell Types STA-PUT Velocity Sedimentation Spermatogenesis Seminiferous Tubules Cell Culture System Tissue Harvest Animal Models Non-spermatogenic Cells Spermatogenic Cells Leydig Cells Sertoli Cells Myeloid Cells Epithelial Cells Spermatogonia Spermatocytes Round Spermatids Condensing Spermatids Spermatozoa Biological Mechanisms Isolation And Enrichment Of Cell Types STA-PUT Method BSA Gradient Sedimentation Velocity Cell Size Fractions Enriched For Different Cell Types
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