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Journal / Bioengineering / Fabricating Complex Culture Substrates Using…
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Bioengineering

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JoVE Journal Bioengineering
Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution
3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening
Journal (Bioengineering)
3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening
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Journal (Bioengineering)
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Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution

Article DOI: 10.3791/52186-v • 08:23 min • October 31st, 2014
October 31st, 2014 •
*1, *1, 1,2, 1
1Department of Biomedical Engineering, University of Wisconsin, Madison, 2Department of Mechanical Engineering, University of Wisconsin, Madison
* These authors contributed equally

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Summary
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Cell culture substrates functionalized with microscale patterns of biological ligands have immense utility in the field of tissue engineering. Here, we demonstrate the versatile and automated manufacture of tissue culture substrates with multiple, micropatterned poly(ethylene glycol) brushes presenting orthogonal chemistries that enable spatially precise and site-specific immobilization of biological ligands.

Tags

Fabricating Complex Culture Substrates Robotic Microcontact Printing Sequential Nucleophilic Substitution Tissue Engineering Spatial Control Tissue Morphology Cell Fate Culture Substrates Grafted Poly(ethylene Glycol) PEG Brushes Microscale Non-fouling Cell Adhesion Resistant Regions Biospecific Interactions Covalently Tethered Ligands Complex Tissues Pattern Multiple PEG Brushes Differential Bioactivities In Vivo Niches Microcontact Printing (& 956;CP) Manual & 956;CP Microscale Precision Advanced Robotics Soft-lithography Techniques Surface Chemistry Reactions Robotic Microcontact Printing (R-& 956;CP) Fabricating Culture Substrates Highly Ordered Patterns Orthogonal & 8216;click& 8217; Chemistries
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