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Construção de-base celular Neurotransmissores fluorescentes Engineered Repórteres (CNiFERs) para detecção óptica de neurotransmissores

Article DOI: 10.3791/53290-v • 12:48 min • May 12th, 2016
May 12th, 2016 •
1, 2, 1, 2,3, 1
1Department of Neuroscience, Icahn School of Medicine at Mount Sinai, 2Department of Physics, University of California, San Diego, 3Section of Neurobiology, University of California, San Diego

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Nós apresentamos um protocolo para criar baseada em células neurotransmissor fluorescentes repórteres modificadas (CNiFERs) para a detecção óptica de liberação do neurotransmissor volumétrica.

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Neurociência Edição 111 imagiologia óptica FSCV diálise a transmissão de volume neurotransmissores neuropeptídeos TPLSM biosensor GPCR
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