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Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1
 

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

Article DOI: 10.3791/53417-v 13:15 min February 25th, 2016
February 25th, 2016

Kapitel

Summary

Here, we outline how to study mitochondrial localization of a (cell cycle) kinase, and how to determine its sub-mitochondrial location as well as potential mitochondrial substrates/targets. Forced expression of proteins into the mitochondria provides a useful tool for studying the functional consequences of mitochondrial localization of a protein of interest.

Tags

Keywords: Mitochondrial Localization Nuclear Cell Cycle Kinase Cdk1 Mitochondrial Research Protein Tagging Subcellular Fractionation Sodium Carbonate Extraction Trichloroacetic Acid Precipitation Immunoblotting
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