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Developmental Biology

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JoVE Journal Developmental Biology
Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes
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Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes

Article DOI: 10.3791/54375-v • 10:03 min • November 11th, 2016
November 11th, 2016 •
1,2, 3, 4,5, 3,6
1Department of Surgery, University of Michigan, 2Department of Biochemistry II, Kanazawa Medical University, 3Center for Immunotherapy, Roswell Park Cancer Institute, 4DNAVEC Corporation, 5Department of Ophthalmology, Keio University School of Medicine, 6Department of Surgical Oncology, Roswell Park Cancer Institute

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Summary
Automatic Translation

The goal of this protocol is to show the protocol for reprogramming melanoma tumor-infiltrating lymphocytes into induced pluripotent stem cells.

Tags

Induced Pluripotent Stem Cells IPSCs Melanoma Tumor-infiltrating Lymphocytes Tumor Immunology Cancer Immunotherapy Immune Therapy Metastatic Melanoma T Cell Therapy T Cell Receptor Repertoire Tumor Microenvironment Tumor Material Tumor Specimens Dissociation Kit Single Cell Suspension 70 Micron Strainer
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