Journal
/
Biologie
/
CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations
JoVE Journal
Biologie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Biologie
CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations

CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations

DOI:
10.3791/54401-v

•

08:09 min

•

August 04, 2016

•

, , , , ,
1The School of Medical Sciences,University of New South Wales Australia, 2Neuroscience Research Australia, 3Biomedical Imaging Facility,University of New South Wales Australia

Kapitel

  • 00:05Titel
  • 01:19Transparent Mouse Skin Tissue Preparation
  • 02:32Mouse Skin Biopsy Clearance
  • 04:27Mouse Skin Biopsy Imaging
  • 05:08Results: Single Cell Resolution Protein Expression in Whole Mount Skin Preparations
  • 06:55Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This report describes a CUBIC protocol to clarify full thickness mouse skin biopsies, and visualize protein expression patterns, proliferating cells, and sebocytes at the single cell resolution in 3D. This method enables accurate assessment of skin anatomy and pathology, and of abnormal epidermal phenotypes in genetically modified mouse lines.

Tags

CUBIC ProtocolProtein ExpressionSingle Cell ResolutionWhole Mount Skin PreparationsEpidermal DevelopmentSkin RegenerationSkin DiseaseHair FolliclesSkin BiopsySkin ClearingCUBIC Clearing Solution

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Method for Whole Mount Antibody Staining in Chick

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations

RNA In situ Hybridization in Whole Mount Embryos and Cell Histology Adapted for Marine Elasmobranchs

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia

An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

Read Article