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Journal / Developmental Biology / Visualisering og kvantitativ analyse af…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
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Visualisering og kvantitativ analyse af embryonisk angiogenese i

Article DOI: 10.3791/55652-v • 06:06 min • May 25th, 2017
May 25th, 2017 •
1, 1,2
1Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, 2Department of Anatomy, Brain Research Institute, Yonsei University College of Medicine

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Denne protokol demonstrerer en fluorescensbaseret metode til visualisering af vaskulaturen og kvantificering af dets kompleksitet i Xenopus tropicalis . Blodkar kan billedbehandles minutter efter injektion af et fluorescerende farvestof i et embryos slaghalve efter genetisk og / eller farmakologisk manipulation for at studere kardiovaskulær udvikling in vivo .

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Developmental Biology Vasculogenesis angiogenesis cardiovascular development, Mikroinjektion DiI-AcLDL billeddannelse venekompleksitetsindeks
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