Tiefen Proteom Profilierung durch isobare Kennzeichnung, umfangreiche Flüssigchromatographie-Massenspektrometrie und softwaregestützte Quantifizierung
11,926 Views
•
10:37 min
•
November 15, 2017
DOI:
Summary
Automatically generated
Wir präsentieren Ihnen ein Protokoll, um präzise quantitate Proteine mit Isobaren Kennzeichnung, umfangreiche Fraktionierung, Bioinformatik und Qualitätskontrolle Schritte in Kombination mit Flüssigkeitschromatographie eine Schnittstelle zu einem hochauflösenden Massenspektrometer.
Verwandte Videos
11:53
Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling
Verwandte Videos
16184 Views
09:06
Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
Verwandte Videos
6893 Views
09:35
Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
Verwandte Videos
13818 Views
08:29
Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
Verwandte Videos
6135 Views
09:33
High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
Verwandte Videos
7196 Views
11:19
Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling
Verwandte Videos
16054 Views
07:44
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
Verwandte Videos
12625 Views
09:24
Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
Verwandte Videos
5558 Views
08:08
Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
Verwandte Videos
16126 Views
N/A
High-throughput and Deep-proteome Profiling by 16-plex Tandem Mass Tag Labeling Coupled with Two-dimensional Chromatography and Mass Spectrometry
Verwandte Videos
5478 Views
Read Article
Cite this Article
High, A. A., Tan, H., Pagala, V. R., Niu, M., Cho, J., Wang, X., Bai, B., Peng, J. Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification. J. Vis. Exp. (129), e56474, doi:10.3791/56474 (2017).
Copy