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Journal / Cancer Research / 结合条件敲击和适应球形成测定研究患者衍生胃癌干细胞的干细胞相关基因…
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Cancer Research

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JoVE Journal Cancer Research
结合条件敲击和适应球形成测定研究患者衍生胃癌干细胞的干细胞相关基因
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结合条件敲击和适应球形成测定研究患者衍生胃癌干细胞的干细胞相关基因

Article DOI: 10.3791/60799-v • 09:24 min • May 9th, 2020
May 9th, 2020 •
*1, *1, *1, 1
1Guangdong Key Laboratory of Genome Stability and Human Disease Prevention, Department of Pharmacology and Shenzhen University International Cancer Center, Shenzhen University School of Medicine
* These authors contributed equally

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Summary
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在该协议中，我们提出了一个实验设计，使用条件敲制系统和适应球形分析，以研究聚类对患者衍生GCSC的茎的影响。该协议可以很容易地适应研究在不同类型的CSC的茎相关基因的体外和体内功能。

Tags

癌症研究， 问题 159， 胃癌干细胞， 条件击倒， 球体形成测定， 聚类， 茎
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