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Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay

Article DOI: 10.3791/3633-v • 09:25 min • October 30th, 2011
October 30th, 2011 •
1,2, 1, 1, 1, 1, 1
1Department of Neurosurgery, University of Florida, 2Department of Anatomical Sciences, Shiraz University of Medical Sciences

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This video protocol demonstrates the isolation and expansion of stem like cells from surgically resected human glioblastoma mutliforme (GBM) tumor tissue using the neurosphere assay culture method.

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Isolation Expansion Human Glioblastoma Multiforme Tumor Cells Neurosphere Assay Stem-like Cells Breast Lung Colon Prostate Brain Tumor Initiating Cell Population(s) Tumor Formation Progression Recurrence Therapeutic Approaches Neurosphere Culture Method Surgically Resected Human GBM Tumor Tissue Chemical Digestion Mechanical Dissociation Single Cell Suspension NSA Culture Primary Neurospheres
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