Co-translationale Insertion von Membranproteinen in vorgeformte Nanoscheiben
3,340 Views
•
08:24 min
•
November 19, 2020
DOI:
Summary
Automatically generated
Die kotranslationale Insertion in vorgeformte Nanoscheiben ermöglicht es, zellfrei synthetisierte Membranproteine in definierten Lipidumgebungen ohne Kontakt mit Detergenzien zu untersuchen. Dieses Protokoll beschreibt die Vorbereitung wesentlicher Systemkomponenten und die kritischen Parameter zur Verbesserung der Expressionseffizienz und Probenqualität.
Verwandte Videos
10:49
Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
Verwandte Videos
13227 Views
11:05
Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells
Verwandte Videos
9024 Views
07:31
Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
Verwandte Videos
5949 Views
07:58
Formulation and Characterization of Bioactive Agent Containing Nanodisks
Verwandte Videos
1079 Views
12:25
A Step-by-step Method for the Reconstitution of an ABC Transporter into Nanodisc Lipid Particles
Verwandte Videos
25470 Views
07:10
Dynamic Light-Induced Protein Patterns at Model Membranes
Verwandte Videos
1136 Views
01:28
Protein Translocation Machinery on the ER Membrane
Verwandte Videos
4573 Views
01:20
Cotranslational Protein Translocation
Verwandte Videos
7322 Views
08:24
Co-Translational Insertion of Membrane Proteins into Preformed Nanodiscs
Verwandte Videos
3.3K Views
15:04
PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays
Verwandte Videos
10.8K Views
Read Article
Cite this Article
Levin, R., Koeck, Z., Dötsch, V., Bernhard, F. Co-Translational Insertion of Membrane Proteins into Preformed Nanodiscs. J. Vis. Exp. (165), e61844, doi:10.3791/61844 (2020).
Copy