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JoVE Journal
Immunology and Infection

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Modelo de cocultura sem contato para o estudo da ativação de células imunes inatas durante a infecção por vírus respiratório

Article DOI: 10.3791/62115-v • 07:36 min • February 28th, 2021
February 28th, 2021 •
*1, *1, 1, 2, 3, 1,4, 1,4, 5, 2,6, 1,2,6, 1,6
1Department of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, 2Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 3Haartman Institute, Medicum, University of Helsinki, 4Department of Otolaryngology, Head and Neck Surgery, National University Hospital, National University Health System, 5Agency for Science, Technology and Research (A*STAR), Singapore Immunology Network (SIgN), 6NUHS Infectious Diseases Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore
* These authors contributed equally

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Este protocolo detalha uma investigação das interações iniciais entre células epiteliais nasais infectadas viralmente e ativação celular inata. Subconjuntos individuais de células imunes podem ser distinguidos com base em sua ativação em resposta a infecções virais. Eles podem então ser investigados para determinar seus efeitos sobre as respostas antivirais precoces.

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Imunologia e Infecção Questão 168 Cocultura células epiteliais nasais humanas células imunes inatas células T inatas vírus respiratório Influenza citocinas citometria de fluxo
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