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Journal / Immunology and Infection / Intravitale widefield fluorescentiemicroscopie…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Intravitale widefield fluorescentiemicroscopie van pulmonale microcirculatie bij experimenteel acuut longletsel met behulp van een vacuümgestabiliseerd beeldvormingssysteem
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Intravitale widefield fluorescentiemicroscopie van pulmonale microcirculatie bij experimenteel acuut longletsel met behulp van een vacuümgestabiliseerd beeldvormingssysteem

Article DOI: 10.3791/63733-v • 09:28 min • April 6th, 2022
April 6th, 2022 •
1, 2, 2,3, 4, 5, 6,7, 2,4, 1,2,4,8
1Department of Physiology and Biophysics, Dalhousie University, 2Department of Microbiology and Immunology, Dalhousie University, 3Department of Neuroscience, Dalhousie University, 4Department of Anesthesia, Pain Management, and Perioperative Medicine, Dalhousie University, 5Live Cell Imaging Center, University of Calgary, 6Department of Physiology and Pharmacology, University of Calgary, 7Department of Biochemistry and Molecular Biology, University of Calgary, 8Department of Pharmacology, Dalhousie University

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Intravitale fluorescentiemicroscopie kan worden gebruikt om leukocyten-endotheelinteracties en capillaire perfusie in realtime te bestuderen. Dit protocol beschrijft methoden om deze parameters in de pulmonale microcirculatie in beeld te brengen en te kwantificeren met behulp van een vacuümgestabiliseerd longbeeldvormingssysteem.

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Immunologie en infectie nummer 182
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