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Påvisning af homologe rekombinationsmellemprodukter via nærhedsligering og kvantitativ PCR i Saccharomyces cerevisiae

Article DOI: 10.3791/64240-v • 07:55 min • September 11th, 2022
September 11th, 2022 •
1, 2, 2, 1,3
1Department of Microbiology & Molecular Genetics, University of California, Davis, 2Laboratory of Biology & Modeling of the Cell, École Normale Supérieure de Lyon, 3Department of Molecular & Cellular Biology, University of California, Davis

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D-loop capture (DLC) og D-loop extension (DLE) assays udnytter princippet om nærhedsligation sammen med kvantitativ PCR til at kvantificere D-loop-dannelse, D-loop-udvidelse og produktdannelse på stedet for et inducerbart dobbeltstrenget brud i Saccharomyces cerevisiae.

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