Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Measuring Physiological Responses of Drosophila…
JoVE Journal
Immunology and Infection

Se requiere una suscripción a JoVE para ver este contenido.

JoVE Journal Immunology and Infection
Measuring Physiological Responses of Drosophila Sensory Neurons to Lipid Pheromones Using Live Calcium Imaging
Murine Superficial Lymph Node Surgery
Journal (Immunology and Infection)
Murine Superficial Lymph Node Surgery
Identifying Caspases and their Motifs that Cleave Proteins During Influenza A Virus Infection
Journal (Immunology and Infection)
Identifying Caspases and their Motifs that Cleave Proteins During Influenza A Virus Infection
 

Measuring Physiological Responses of Drosophila Sensory Neurons to Lipid Pheromones Using Live Calcium Imaging

Article DOI: 10.3791/53392-v • 07:25 min • April 29th, 2016
April 29th, 2016 •
1,2, 3,4, 1,2,5
1Temasek Life Sciences Laboratory, 2Department of Biological Science, National University of Singapore, 3Bioimaging and Biocomputing Facility, Temasek Life Sciences Laboratory, 4Histology and Light Microscopy Core, Gladstone Institutes, 5Pacific Biosciences Research Center, University of Hawai'i at Mānoa

Capítulos

Resumen
Automatic Translation

The forelegs and proboscis of Drosophila contain a rich repertoire of gustatory sensory neurons. Here, we present a method using calcium imaging to measure physiological responses from sensory neurons in the foreleg and proboscis of live flies upon exogenous application of a gustatory pheromone.

Tags

Physiological Responses Drosophila Sensory Neurons Lipid Pheromones Live Calcium Imaging Gustatory Receptors Sensory Neurobiology Intact Live Animal Anesthetizing Cover Slip Clear Nail Polish Dissecting Microscope Foreleg Tarsal Segments Tape Proboscis Labellum PAP Pen Anesthesia Wear Off Measurements Stock Ligand Solution PBST
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter