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Development of a Colloidal Gold-based Immunochromatographic Test Strip for Detection of Cetacean Myoglobin

Article DOI: 10.3791/53433-v • 11:48 min • July 13th, 2016
July 13th, 2016 •
1, 1, 2, 2, 1, 1
1Department of Veterinary Medicine, National Chiayi University, 2Department of Microbiology and Immunology/Graduate Institute of Biomedical and Biopharmaceutical Sciences, National Chiayi University

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This protocol describes the development of two IgG class monoclonal antibodies (mAbs) strongly reactive to myoglobin of cetaceans. These mAbs are applied on a colloidal gold immunochromatographic test strip based on the sandwich format to differentiate the Mb of cetaceans from seal and other animals.

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Colloidal Gold-based Immunochromatographic Test Strip Cetacean Myoglobin Detection Rapid Screening Illegal Trade Prevention Specificity And Sensitivity Synthetic Peptides Monoclonal Antibodies Amino Acid Sequences GenBank Retrieval Alignment Analysis
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